SEQUENCE OF CHICKEN C-BETA-7 TUBULIN ANALYSIS OF A COMPLETE SET OF VERTEBRATE BETA-TUBULIN ISOTYPES

[摘要] In chicken, .beta.-tubulin is encoded by a family of seven genes. We have now isolated and sequenced overlapping cDNA clones corresponding to gene c.beta.7 (previously designated c.beta.4''), the only chicken .beta.-tubulin not previously characterized. The inferred amino acid sequence of c.beta.7 tubulin is identical with the class I .beta.-tubulin isotype found in human, mouse and rat. Moreover, c.beta.7 is highly expressed in almost all tissue and cell types in chicken, a pattern similar to those of the genes for class I .beta.-tubulin isotypes in other vertebrates. Comparison of the complete family of chicken .beta.-tubulin gene sequences reveals that the heterogeneity of .beta.-tubulin polypeptides encoded in a higher eukaryote is confined to six distinct .beta.-tubulin isotypes. Five of these are members of evolutionarily conserved isotypic classes (I to V), whereas the six represents a divergent erythroid-specific tubulin whose sequence has not been conserved.

[发布日期] 1988-02-05