A mutation in the S-switch region of the runt domain alters the dynamics of an allosteric network responsible for CBFβ regulation

[摘要] The Runt domain is the DNA binding domain of the core binding factor (CBF) Runx subunits. The CBFs are transcription factors that play critical roles in hematopoiesis, bone, and neuron development in mammals. A common non-DNA binding CBF beta subunit heterodimerizes with the Runt domain of the Runx proteins and. allosterically regulates its affinity for DNA. Previous NMR dynamics studies suggested a model whereby CBF beta allosterically regulates DNA binding by quenching conformational exchange in the Runt domain, particularly in the S-switch region and the beta E'-F loop. We sought to test this model, and to this end introduced all possible single amino acid substitutions into the S-switch region and the beta E'-F loop, and screened for mutations that enhanced DNA-binding. We demonstrate that one Runt domain mutant, R164N, binds both DNA and CBF beta with higher affinity, but it is less sensitive to allosteric regulation by CBF beta. Analysis of NMR relaxation data shows that the chemical exchange exhibited by the wild-type Runt domain is largely quenched by the R164N substitution. These data support a model in which the dynamic behavior of a network of residues connecting the CBF beta and DNA binding sites on the Runt domain plays a critical role in the mechanism of allosteric regulation. This study provides an important functional link between dynamic behavior and protein allosteric function, consistent with results on other allosterically regulated proteins. (c) 2006 Elsevier Ltd. All rights reserved.

[发布日期] 2006-12-15

[关键词] RUNX;AML1;CBF beta;allostery;NMR