Structural Features that Distinguish Inactive and Active PI3K Lipid Kinases

[摘要] PI3K lipid kinases signal through the PI3K/Akt pathway, regulating cell growth and proliferation. While the structural features that distinguish between the active and inactive states of protein kinases are well established, that has not been the case for lipid kinases, and neither was the structural mechanism controlling the switch between the two states. Class I PI3Ks are obligate heterodimers with catalytic and regulatory subunits. Here, we analyze PI3K crystal structures. Structures with the nSH2 (inactive state) are featured by collapsed activation loop (a-loop) and an IN kinase domain helix 11 (k alpha 11). In the active state, the a-loop is extended and k alpha 11 in the OUT conformation. Our analysis suggests that the nSH2 domain in the regulatory subunit regulates activation, catalysis and autoinhibition through the a-loop. Inhibition, activation and catalytic scenarios are shared by class IA PI3Ks; the activation is mimicked by oncogenic mutations and the inhibition offers an allosteric inhibitor strategy. (C) 2020 The Author(s). Published by Elsevier Ltd.

[发布日期] 2020-11-06

[关键词] PI3K alpha;Ras;nSH2;catalytic and regulatory subunits;PI3K mutations