NUCLEOTIDE-SEQUENCE OF THE TRPC-TRPB INTERCISTRONIC REGION FROM SALMONELLA-TYPHIMURIUM

[摘要] A DNA segment that contains the S. typhimurium trpC-trpB junction was sequenced. A series of 11 amino acids predicted from the sequence are identical to the NH3-terminal amino acid sequence of Escherichia coli tryptophan synthetase .beta.. Carboxypeptidase A digestion of phosphoribosyl-anthranilate isomerase-indoleglycerolphosphate synthetase identified its carboxy-terminal amino acids, allowing us to specify the end of trpC. Nine nucleotides separate the terminator codon of trpC from the initiator codon of trpB. The mRNA around the trpB initiation site, and around many other prokaryotic ribosome binding sites, has the potential to form stable stem and loop structures. These secondary structures share the property of having most, if not all, of the sequences complementary to the 3'' end of 16 S rRNA and the initiator codon, included in single-stranded regions.