被撤回的出版物: MicroRNA-498 disturbs the occurrence and aggression of colon cancer through targeting MDM2 to mediate PPARγ ubiquitination (Retracted article. See vol. 319, 2023)

[摘要] Objective: MicroRNA (miR)-498 is indicative of diagnostic and prognostic significance in colon cancer (CC). On the basis of that, this study is initiated from miR-498, combined with mouse double minute 2 (MDM2)/peroxisome proliferator-activated receptor gamma (PPAR gamma) ubiquitination axis to have an insight into CC progression. Methods: CC tissues and their adjacent tissues were harvested to determine miR-498, MDM2 and PPAR gamma expression. The interactions among these three factors were identified. The screened human CC cells were transfected with miR-498/MDM2-related sequences, followed by detection of the biological behaviors of CC cells. Xenografted tumors were taken to validate cell experimental outcomes. Bioinformatics and dual-luciferase report analysis verified the targeting relationship between miR-498 and MDM2. The relation between MDM2 and PPAR gamma was identified by immunoprecipitation and in vivo deubiquitination. Results: Down-regulated miR-498 and PPAR gamma and up-regulated MDM2 were exhibited in CC. miR-498 targeted MDM2 while MDM2 mediated PPAR gamma ubiquitination. Elevated miR-498 or reduced MDM2 impaired cell viability, colony-forming, migratory and invasive activities and enhanced apoptosis in CC. Elevated MDM2 abolished the effects of up-regulated miR-498 on the biological behaviors of CC cells. Elevated miR-498 or reduced MDM2 depressed tumorigenic ability of CC cells in mice. Conclusion: It is conclusive that restoring miR-498 depresses MDM2 to modify PPAR gamma ubiquitination, thereby disturbing the tumorigenesis of CC. This work constructs the base for exploring novel agents in treating CC.

[发布日期] 2021-07-15

[效力级别]  Retracted Publication

[关键词] Colon cancer;MicroRNA-498;Mouse double minute 2;Peroxisome proliferator-activated receptor gamma;Ubiquitination;Tumorigenesis