A G(i)2 alpha antisense oligonucleotide differentiates morphine antinociception, constipation and acute dependence in mice

[摘要] In the same mice in which the intracerebroventricular (i.c.v.) administration of antisense oligodeoxyribonucleotide (oligo) directed against the G(i)2 alpha (but not G(i)1 alpha, G(i)3 alpha or G(s) alpha) G-protein subunits attenuated i.c.v. morphine-induced antinociception in the tail-flick test, none of the oligos altered naloxone-precipitated jumping (acute dependence). Likewise, none of the oligos significantly altered morphine-induced constipation. Hence; i.c.v. morphine-induced antinociception might be preferentially mediated via transduction pathway(s) different from constipation or acute dependence, offering novel opportunities for drug discovery.

[发布日期] 1995-12-22

[关键词] antisense;G-proteins;morphine;analgesia;constipation;dependence