The impact of periodontitis in the course of chronic obstructive pulmonary disease: Pulmonary and systemic effects

[摘要] Aims: The aim of this study was to verify the impact of periodontitis in the course of chronic obstructive pulmonary disease (COPD) in C57B1/6J mice. Main methods: The animals were randomly divided into four groups (n = 8): Basal, Periodontitis (P), COPD and COPD + P. COPD was induced by orotracheal instillation of 30 mu l of cigarette extract 3 times/week for 7 weeks. Periodontitis was induced by ligation technique for 22 days. Euthanasia was performed on 51st day. The analyzes were total/differential cells and cytokines recovered from bronchoalveolar lavage (BAL), total/differential blood cell count, platelets, total marrow cell count, airway collagen deposition, alveolar enlargement analyzed by mean linear intercept (Lm), mucus and bone crest reabsorption. One-way ANOVA followed by the Student-Newman-Keuls was used. Key findings: The association COPD + P decreased macrophages (p = 0,0351), TNF-alpha (p = 0,0071) and INF-gamma (p = 0,0004) in BAL, when compared to the COPD group maintaining emphysema levels by alveolar enlargement (p < .05) reorganization of collagen fibers (p = .001) and also mean linear intercept (lm) (p = .001) and mucus (p = .0001). The periodontitis group caused TNF-alpha increase (p = 0, 0001) in BAL. Significance: Periodontitis, per se, does not alter any of the parameters analyzed, except for increased TNF-alpha in BAL. However, its association with COPD caused macrophages TNF-alpha and INF-gamma alterations, when compared to the COPD group maintaining emphysema levels by alveolar enlargement and reorganization of collagen fibers. It seems that periodontitis is influencing the course of Th1 profile cell, and cytokines and pulmonary alterations. Further studies are needed to clarify the regulatory process underlying these two diseases.

[发布日期] 2020-11-15

[关键词] Bronchoalveolar lavage;Cytokines;Blood cell count;Pulmonary