Surface probe measurements of the elasticity of sectioned tissue, thin gels and polyelectrolyte multilayer films: Correlations between substrate stiffness and cell adhesion

[摘要] Surface probe measurements of the elasticity of thin film matrices as well as biological samples prove generally important to understanding cell attachment across such systems. To illustrate this, sectioned arteries were probed by atomic force microscopy (AFM) within the smooth muscle cell (SMC)-rich medial layer, yielding an apparent Young's modulus E-media similar to 5-8 kPa. Polyacrylamide gels with E-gel spanning several-fold above and below this range were then cast 5-70 [mum thick and coated with collagen: SMC spreading shows a hyperbolic dependence in projected cell area versus E-gel. The modulus that gives half-max spreading is E1/2-spread similar to 8-10 kPa, proving remarkably close to E,(media). More complex, layer-by-layer microfilms of poly((L)-lysine)/hyaluronic acid were also tested and show equivalent trends of increased SMC spreading with increased stiffness. Adhesive spreading of cells thus seems to correlate broadly with the effective stiffness of synthetic materials and tissues. (C) 2004 Elsevier B.V. All rights reserved.

[发布日期] 2004-10-10

[效力级别]  Proceedings Paper

[关键词] surface structure, morphology, roughness and topography;adhesion;atomic force microscopy