STUDIES ON THE MOLECULAR REGULATION OF THE INDUCIBLE FORM OF NITRIC-OXIDE SYNTHASE (INOS) IN INSULIN-PRODUCING CELLS

[摘要] Nitric oxide, a radical generated by the enzyme nitric oxide synthase (iNOS), may be an important mediator of beta-cell damage in early insulin-dependent diabetes mellitus. We have investigated the molecular regulation of iNOS in insulin-producing RINm5F cells. The data obtained suggest that iNOS is maximally induced in these cells by a 6-h exposure to IL-1 beta or TNF-alpha + IFN-gamma, but not by endotoxin. iNOS mRNA degradation is rapid and it is not affected by IL-1 beta. Interestingly, NO seems to induce a negative feedback on iNOS expression, probably by decreasing iNOS transcription.

[发布日期] 1994-12-01

[关键词] NITRIC OXIDE;NITRIC OXIDE SYNTHASE;PANCREATIC BETA-CELLS;CYTOKINES;INTERLEUKIN-1-BETA;INTERFERON-GAMMA;TUMOR NECROSIS FACTOR-ALPHA