EXPRESSION OF S14-MESSENGER-RNA AND ITS TRANSLATIONAL PRODUCT IN DIFFERENTIATING 3T3-L1 CELLS

[摘要] During adipogenic conversion of 3T3-L1 cells S14-mRNA increased from undetectable levels in preadipocytes to high levels in differentiated adipocytes (adipocyte-like cells). In vitro translation of hybrid-selected S14-mRNA revealed an identical protein in 3T3-L1 adipocytes and in rat liver with regard to migration properties in two-dimensional gel electrophoresis. No translation product was found in 3T3-L1 preadipocytes. In conclusion, protein-S14 is very similar or even identical in rat lipogenic tissues and in 3T3-L1 adipocytes. Therefore, this cell line can be employed to elucidate further physiological aspects of protein-S14.

[发布日期] 1991-03-01

[关键词] PROTEIN-S14;ADIPOGENIC DIFFERENTIATION;3T3-L1 CELL;HYBRID SELECTION;TRANSLATION;INVITRO;2-DIMENSIONAL GEL ELECTROPHORESIS