Ethanol exerts anti-inflammatory effects in human adipose tissue in vitro

[摘要] Moderate alcohol consumption is suggested to be associated with reduced inflammation and morbidity. Human adipose tissue (AT) and obesity is characterised by low-grade inflammation, so the present study wanted to investigate the effects of ethanol on inflammation in human AT in vitro. Subcutaneous human AT was incubated with ethanol [11-88 mM] under non-or LPS-stimulated [50 mg/mL] conditions. Protein and mRNA levels of adiponectin, IL-6, IL-8, TNF-alpha, MCP-1, and CD68 were assessed using ELISA and real-time RT-PCR, respectively. Non-stimulated. ethanol incubations up to 24h increased adiponectin release and mRNA expression (p<0.01) and decreased IL-6 release in both short-term [1.5h] (p<0.05) and long-term [24h] (p<0.01)incubations. Ethanol decreased LPS-stimulated IL-6, IL-8,TNF-alpha, and MCP-1 dose-dependently (all p < 0.01). Ethanol decreased CD68 mRNA (p, < 0.001),which correlated with the investigated adipokines (p < 0.05) but not adiponectin (p > 0.05). In conclusion. ethanol exerts anti-inflammatory effects in human AT, Suggesting that ethanol may attenuate whole-body inflammation. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

[发布日期] 2008-12-16

[关键词] Ethanol;Adipokines;Inflammation;Adipose tissue (human);Macrophages