Recognition of a human sperm surface protein involved in the progesterone-initiated acrosome reaction by antisera against an endomembrane progesterone binding protein from porcine liver

[摘要] Antisera against a porcine liver endomembrane progesterone (P-4)-binding protein inhibited the P-4-initiated acrosome reaction (AR) but not the ionomycin-initiated AR of human sperm. Indirect: immunofluorescence studies detected antigen in the sperm head that moved during capacitation from a posterior head region to a midhead region. Moreover, the antisera detected a 44.6 kDa protein in western blots of sperm digitonin extracts. These results suggest that a sperm protein with at least partial homology to the liver endomembrane P-4-binding protein, is a putative P-4-receptor on the sperm plasma membrane involved in the P-4-initiated AR. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

[发布日期] 1999-12-20

[关键词] sperm;progesterone;liver (P-4)-binding protein;antisera;acrosome reaction