Kartogenin Enhances Collagen Organization and Mechanical Strength of the Repaired Enthesis in a Murine Model of Rotator Cuff Repair

[摘要] Purpose: To investigate the use of kartogenin (KGN) in augmenting healing of the repaired enthesis after rotator cuff repair in a murine model. Methods: Seventy-two C57BL/6 wild-type mice underwent unilateral detachment and transosseous repair of the supraspinatus tendon augmented with either fibrin sealant (control group; n = 36) or fibrin sealant containing 100 mmol/L of KGN (experimental group; n = 36) applied at the repair site. Postoperatively, mice were allowed free cage activity without immobilization. Mice were humanely killed at 2 and 4 weeks postoperatively. Repair site integrity was evaluated histologically through fibrocartilage formation and collagen fiber organization and biomechanically through load-to-failure testing of the supraspinatus tendon-bone construct. Results: At 2 weeks, no differences were noted in percent area of fibrocartilage, collagen organization, or ultimate strength between groups. At 4 weeks, superior collagen fiber organization (based on collagen birefringence [17.3 +/- 2.0 vs 7.0 +/- 6.5 integrated density/mu m(2); P < .01]) and higher ultimate failure loads (3.5 +/- 0.6 N vs 2.3 +/- 1.1 N; P = 04) were seen in the KGN group. The percent area of fibrocartilage (13.2 +/- 8.4% vs 4.4 +/- 5.4%; P = .04) was higher in the control group compared with the KGN group. Conclusions: Rotator cuff repair augmentation with KGN improved the collagen fiber organization and biomechanical strength of the tendon-bone interface at 4 weeks in a murine model.

[发布日期] 2018-09-01

[效力级别]  Proceedings Paper