Integration of a flow-type chemiluminescence detector on a glass electrophoresis chip

[摘要] A glass electrophoresis microchip integrated a flow-type chemluminescence (CL) detection cell has been developed and evaluated. The chip pattern is a double-T-type electrophoretic sample injection and separation combining with a Y-type chemiluminecent detector. The double-T geometry allows for high-efficiency sample injection and geometric definition of sample plug size. The branch of Y was used as CL reagent channel, and the CL reagent was delivered by a tab-made micropump. Bis[(2,4,6-trichlorophenyl)]oxalate-H2O2 CL system was employed to detect dansyl amino acids. On this microchip, dansyl-phenylalanine and -sarcosine were successfully separated by electrophoresis and detected within 250 s. The detection limits (S/N = 3) of dansyl-phenylalanine and -sarcosine could reach to 2.8 and 3.2 muM, respectively, due to the vigorous dilution of sample with CL reagent and timely removal of the waste solution from reaction area. (C) 2004 Elsevier B.V. All rights reserved.

[发布日期] 2004-11-15

[关键词] microchip electrophoresis;chemiluminescence detection;flow-type detection cell;double-T injection