CHORISMATE MUTASE PREPHENATE DEHYDRATASE FROM ESCHERICHIA-COLI - SUBCLONING, OVERPRODUCTION AND PURIFICATION

[摘要] Efficient overproduction of chorismate mutase/prephenate dehydratase (20% of soluble protein) has been achieved using recombinant DNA techniques. A new purification procedure utilizing hydrophobic and ion-exchange chromatography yielded homogeneous enzyme in two steps.

[发布日期] 1991-04-08