Identification of a novel oxygenase capable of regiospecific hydroxylation of D-limonene into (+)-trans-carveol

[摘要] By genome sequencing and analysis, we have been able to identify a gene cluster encoding a four component oxygenase which is able to oxidize D-limonene, L-limonene, cumene, and indole. Heterologous expression of the complete oxygenase system in Pseudomonas putida S12 allowed efficient and highly regiospecific biotransformation of D-limonene into (+)-trans-carveol. The discovered oxygenase represents a promising biocatalyst for the biotechnological valorisation of D-limonene. (C) 2015 Elsevier Ltd. All rights reserved.

[发布日期] 2016-11-17

[效力级别]  Proceedings Paper

[关键词] D-Limone;Carveol;Carvone;Cumene;Oxygenase;Hydroxylation