Overexpression, purification and characterization of two pyrimidine kinases involved in the biosynthesis of thiamin: 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase and 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase

[摘要] The overexpression, purification and characterization of 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase (HMP kinase) and 4-amino-5-hydroxymethyl-2-methylpyrimidine monophosphate kinase (HMP-P kinase) are described, Surprisingly HMP-P kinase also shows HMP kinase activity, These enzymes are useful reagents for the preparation of intermediates on the thiamin biosynthetic pathway. (C) 1998 Elsevier Science Ltd. All rights reserved.

[发布日期] 1998-12-24

[关键词] enzymes;phosphorylation;biosynthesis