Rapid and sensitive detection of norovirus antibodies in human serum with a biolayer interferometry biosensor

[摘要] Here, we describe the use of a biolayer interferometry biosensor for the fast and sensitive detection of virus-specific antibodies from human serum samples. Norovirus-like particles and norovirus P-particles were used to functionalise the biosensor tip. The detection of antibodies directly from serum samples was challenging, but the addition of a metal chelator (DAB) combined with an anti-human horseradish peroxidase-tagged antibody enabled enhanced detection of virus-specific antibodies in serum dilutions up to 1:100,000. Biolayer interferometry provides results faster than an ELISA, with results in as little as 10-20 min when using pre-functionalised sensors. Therefore, biolayer interferometry combined with DAB enhancement offers an attractive method for quick and sensitive quantification of biomolecules from complicated sample matrices. (C) 2015 Elsevier B.V. All rights reserved.

[发布日期] 2015-12-31

[关键词] Biolayer interferometry;Norovirus;Virus-like particles (VLPs);P-particles;Non-labelled detection;Fast diagnostics