Needle-type dissolved oxygen microelectrode array sensors for in situ measurements

[摘要] Dissolved oxygen is a critical indicator in a variety of biomedical and environmental processes. This work describes the development of a new needle-type microelectrode array (MEA) sensor for in situ measurements of dissolved oxygen (DO) using microfabrication technologies. A dynamic etching technique was used to fabricate 10mm long sensor probes and sharpen them to micrometer dimensions. The sensors utilized a gold sensing electrode inside a recess fabricated at the tip of each microelectrode. Electrochemical performance of these DO MEA sensors was fully characterized by measuring the oxygen concentration of saline solutions with a Ag/AgCl reference electrode. The DO MEA exhibited a rapid 15 s linear response in the 0-9 mg/L (0-21% O-2) range. The DO microelectrode array sensor was successfully applied to evaluation of DO microprofiles in a multi-species aerobic bacterial film. The developed new sensors are able to penetrate samples in order to perform DO measurements, and will enable in situ analysis in many biological applications. (c) 2007 Elsevier B.V. All rights reserved.

[发布日期] 2007-12-12

[关键词] dissolved oxygen;oxygen sensor;microelectrode array;needle-type sensor