Immune alterations in male and female mice after 2-deoxy-D-glucose administration

[摘要] Administration of 2-deoxy-D-glucose (2-DG) induces acute cellular glucoprivation. In the current study, we examined differences in immune parameters after 2-DG administration in both sexes. Male and female BDF, mice were injected three times, 48 h apart, either with a saline solution (control group) or with 2-DG in saline (500 mg/kg). Two hours after the last injection, blood and spleens were collected. Plasma levels of interleukin-lp, and interferon-gamma levels were measured. Additionally, the levels of the specific leukocyte antigens CD3, CD4, CD8, T cell receptor (TCR) alpha/beta, I-A(d), and H-2L(d)/H-2D(b) were evaluated by flow cytometry on both blood and spleen cells. The blastogenic response of leukocytes from both tissues to mitogens was assessed. Levels of glucose, corticosterone, testosterone, progesterone, 17 beta-estradiol, follicle-stimulating hormone, and luteinizing hormone were also determined. Increases in the percentage of cells bearing TCR odp and I-Ad in the blood and H-2L(d)/H-2D(b) in the spleen were observed in the 2-DG-treated group for both sexes. In contrast, higher corticosterone and IL-1 beta plasma concentrations, as well as higher percentages of splenocytes bearing TCR alpha/beta and I-A(d), and lower mitogen-induced proliferation of mature T splenocytes (79%) were observed in female but not in male mice injected with 2-DG compared with those injected with saline (p < 0.05). Taken together, these results suggest that female mice are more sensitive than male mice to immune alterations induced by 2-DG administration. (C) 1997 Elsevier Science Inc.

[发布日期] 1997-12-01

[关键词] 2-deoxy-D-glucose;corticosterone;interleukin;lymphocyte;metabolism;sex