(S)-[18F]THK5117 brain uptake is associated with Aβ plaques and MAO-B enzyme in a mouse model of Alzheimer's disease

[摘要] The mouse model of beta-amyloid (A beta) deposition, APP/PS1-21, exhibits high brain uptake of the tau-tracer (S)[18F]THK5117, although no neurofibrillary tangles are present in this mouse model. For this reason we investigated (S)-[18F]THK5117 off-target binding to A beta plaques and MAO-B enzyme in APP/PS1-21 transgenic (TG) mouse model of A beta deposition. APP/PS1-21 TG and wild-type (WT) control mice in four different age groups (2-26 months) were imaged antemortem by positron emission tomography with (S)-[18F]THK5117, and then brain autoradiography. Additional animals were used for immunohistochemical staining and MAO-B enzyme blocking study with deprenyl pre-treatment. Regional standardized uptake value ratios for the cerebellum revealed a significant temporal increase in (S)-[18F]THK5117 uptake in aged TG, but not WT, brain. Immunohistochemical staining revealed a similar increase in A beta plaques but not endogenous hyper-phosphorylated tau or MAO-B enzyme, and ex vivo autography showed that uptake of (S)-[18F]THK5117 co-localized with the amyloid pathology. Deprenyl hydrochloride pre-treatment reduced the binding of (S)-[18F]THK5117 in the neocortex, hippocampus, and thalamus. This study's findings suggest that increased (S)-[18F]THK5117 binding in aging APP/PS1-21 TG mice is mainly due to increasing A beta deposition, and to a lesser extent binding to MAO-B enzyme, but not hyper-phosphorylated tau.

[发布日期] 2021-09-15

[关键词] Alzheimer's disease;APP;PS1-21;Beta-amyloid;MAO-B;(S)-[F-18]THK5117;Tau