ONE-POT SELECTIVE DEUTERIATION OF 5'-DIMETHOXYTRITYLATED DEOXYNUCLEOTIDE DERIVATIVES

[摘要] Procedures have been developed for selective incorporation of deuterium at C-6 of 5'-DMT-dC(Bz)@, C-8 of 5'-DMT-dA(Bz), C-8 of 5'-DMT-dG(i-Bu), and C-6 of 5'-DMT-dT in dimethylsulfoxide solution catalyzed by various amounts of base at 90-135-degrees-C with quantitative or nearly quantitative deuterium-labeling yields. The reactions of deuterium exchange of C-6 of 5'-DMT-dC(Bz), C-8 of 5'-DMT-dA(Bz), and C-8 of 5'-DMT-dG(i-Bu) were performed in a one-pot reaction procedure and that of C-6 of 5'-DMT-dT was achieved by deuteriation of 5'-unprotected dT, followed by dimethoxytritylation of 5'-OH with 4,4'-dimethoxytrityl chloride.

[发布日期] 1994-03-24