Real-time voltammetric detection of cocaine-induced dopamine changes in the striatum of freely moving mice

[摘要] In the present voltammetric study, we have characterized cocaine-induced changes in evoked dopamine release and uptake in the striatum of freely moving mice in real time. Cocaine induced marked dopamine uptake inhibition measured as apparent K-m changes, producing a maximal effect 20 min following a single injection (15 mg/kg, i.p.). Changes in uptake were paralleled by increases in evoked dopamine release per stimulus pulse, revealing a high correlation between these two parameters following cocaine administration. This initial characterization of cocaine effects on striatal dopamine transmission in the commonly used C57BL/6 mouse strain provides a basis for future voltammetric studies using genetic mouse models. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

[发布日期] 2009-12-25

[关键词] Cocaine;Freely moving mouse;Voltammetry;Dopamine