CALCIUM-DEPENDENT RELEASE OF ACCUMULATED GLUTAMATE FROM SYNAPTIC VESICLES WITHIN PERMEABILIZED NERVE-TERMINALS

[摘要] We have studied glutamate release from synaptic vesicles in permeabilized synaptosomes, which were preloaded with [H-3]glutamate in an ATP-dependent manner. The release was found to be calcium-dependent and to require a heat-labile cytosolic macromolecule factor for maximum activity. Maximal release occurred at 5-mu-M free Ca2+ and within 5 min. Of the other divalent cations tested, only barium stimulated release of vesicular glutamate. The release was inhibited by N-ethylmaleimide. These results are characteristic of exocytotic release of monoamines and peptides observed in endocrine systems, and constitute direct evidence for the notion that calcium-dependent release of glutamate originates from the vesicular pool.

[发布日期] 1991-01-28

[关键词] SYNAPTIC VESICLE;NEUROTRANSMITTER RELEASE;CALCIUM-DEPENDENT;GLUTAMATE;ATP-DEPENDENT UPTAKE