PROCESSING OF PRO-OPIOMELANOCORTIN-DERIVED AMIDATED JOINING PEPTIDE AND GLYCINE-EXTENDED PRECURSOR IN MONKEY PITUITARY

[摘要] The molecular forms of proopiomelanocortin (POMC) derived amidated and C-terminal glycine-extended joining peptide from monkey (Macaca mulatta) pituitary were determined. The predominant forms of joining peptide found were the low molecular peptides POMC(76-105) and POMC(76-106), respectively. Significant amounts of N-terminally truncated POMC(78-105) and POMC(78-106) were also detected in the posterior-intermediate lobe. No N-terminal extended froms were detected. The relative amount of amidated joining peptide to total joining peptide was 6-35%. It is concluded that not only is the primary sequence of monkey and human POMC extremely conserved, but also the processing patterns are similar. The monkey therefore serves as a suitable model for studying regulation of the processing of POMC and the hypothalamus-pituitary-adrenal axis in man.

[发布日期] 1991-04-01

[关键词] PROOPIOMELANOCORTIN;JOINING PEPTIDE;MONKEY;PRIMATE;AMIDATION;GLYCINE-EXTENDED;PITUITARY;ACTH