A CHLORIDE CHANNEL IN RAT AND HUMAN AXONS

[摘要] Current recordings from single chloride channels were obtained from excised and cell-attached patches of rat and human axons. In rat axons the channels showed an outwardly rectifying current-voltage relationship with a slope conductance of 33 pS at negative membrane potentials and 65 pS at positive potentials (symmetrical 150 mM CsCl). They were measurably permeable for cations (P(Na)/P(Cs)/P(Cl) = 0.1/0.2/1). Channel currents were independent of cytoplasmatic calcium concentration. Inactivation was not observed and gating was weakly voltage dependent. Cl- channels in human axons showed similar gating behavior but had a lower conductance.

[发布日期] 1991-12-09

[关键词] AXON;MYELINATED FIBER;PATCH CLAMP;SINGLE CHLORIDE CHANNEL;BACKGROUND CONDUCTANCE;RAT;HUMAN