TETRAHYDROAMINOACRIDINE (THA) REDUCES VOLTAGE-DEPENDENT CALCIUM CURRENTS IN RAT SENSORY NEURONS

[摘要] Tetrahydroaminoacridine (THA) is a centrally active anticholinesterase that also interacts with neuronal K+ and Na+ channels and cardiac Ca2+ channels. The effects of THA on neuronal voltage-dependent Ca2+ channels are not known. We tested the effects of THA (25 nM-250-mu-M) on the Ca2+ current components of acutely dissociated rat nodose ganglion and dorsal root ganglion (DRG) neurons using the whole cell patch clamp recording technique. THA reduced the low-threshold (T) and high-threshold (N/L) Ca2+ current components in a concentration-dependent manner (IC50 congruent-to 125-mu-M for T; congruent-to 80-mu-M for N/L). Minimal current reduction was seen below approximately 10-mu-M. Our results show that THA reduces voltage-dependent Ca2+ currents in rodent sensory neurons suggesting another means by which THA may affect Ca2+-dependent physiologic processes.

[发布日期] 1991-11-11

[关键词] TETRAHYDROAMINOACRIDINE;TACRINE;ALZHEIMERS DISEASE;CALCIUM CURRENT;WHOLE CELL PATCH CLAMP