Cloning and characterization of the murine homologue of the rat/human MRC OX-2 gene

[摘要] A 350bp amplicon, obtained by PCR-select subtractive hybridization from RNA derived from mesenteric lymph nodes (MLN) cells from mice pre-immunized with allogeneic lymphocytes 36hrs prior to receiving donor-specific skin grafts, and showing >98% homology with a published sequence for the rat MRC OX-2 gene, was used as a hybridization probe to screen a cDNA library constructed from adult mouse MLN treated in the same fashion. Several clones were identified which, on DNA sequence analysis, predicted a 218 amino acid protein showing significant homology with the rat and human MRC OX-2 gene product. (C) 1997 Elsevier Science B.V.

[发布日期] 1997-11-28

[关键词] MRC OX-2;tolerance;immunoregulation;transplantation