Immobilized microorganisms in the reduction of ethyl benzoylacetate

[摘要] The enantioselective reduction of ethyl benzoylacetate (EBA) into ethyl (S)-3-hydroxy-3-phenylpropanoate (S-HPPE) by nine yeast strains and three filamentous fungi strains is described. The conversion obtained was in the range 0-89% and the enantiomeric excess was 100% in many cases. Conversion levels were higher when the reduction was performed with microorganisms immobilized in Calcium alginate and enantioselectivity remained excellent. Some reaction's conditions of bioreduction by immobilized cells of Rhodotorula rubra were studied using a 2(5-2) fractional factorial design. (C) 2009 Elsevier Ltd. All rights reserved.

[发布日期] 2009-12-30

[关键词] beta-Ketoester;Immobilization;Rhodotorula rubra;Chiral reduction