CRISPR–Cas system to discover host-virus interactions in Flaviviridae

[摘要] The Flaviviridae virus family members cause severe human diseases and are responsible for considerable mortality and morbidity worldwide. Therefore, researchers have conducted genetic screens to enhance insight into viral dependency and develop potential anti-viral strategies to treat and prevent these infections. The host factors identified by the clustered regularly interspaced short palindromic repeats (CRISPR) system can be potential targets for drug development. Meanwhile, CRISPR technology can be efficiently used to treat viral diseases as it targets both DNA and RNA. This paper discusses the host factors related to the life cycle of viruses of this family that were recently discovered using the CRISPR system. It also explores the role of immune factors and recent advances in gene editing in treating flavivirus-related diseases. The ever-increasing advancements of this technology may promise new therapeutic approaches with unique capabilities, surpassing the traditional methods of drug production and treatment.

[发布日期] 2023-10-25

[关键词] Flaviviridae pathogenesis;CRISPR/Cas;Antiviral strategy;Flavivirus;Host factors;Genome editing;CRISPR KO;Virus-host interactions;Genome-wide CRISPR screens