Quality assessment and optimization of purified protein samples: why and how?

[摘要] Purified protein quality control is the final and critical check-point of any protein production process. Unfortunately, it is too often overlooked and performed hastily, resulting in irreproducible and misleading observations in downstream applications. In this review, we aim at proposing a simple-to-follow workflow based on an ensemble of widely available physico-chemical technologies, to assess sequentially the essential properties of any protein sample: purity and integrity, homogeneity and activity. Approaches are then suggested to optimize the homogeneity, time-stability and storage conditions of purified protein preparations, as well as methods to rapidly evaluate their reproducibility and lot-to-lot consistency.

[发布日期] 2014-12-30

[关键词] Recombinant protein;Therapeutic;Diagnosis;Structural biology;Electrophoresis;Mass spectrometry;UV/visible spectroscopy;Light scattering;Size-exclusion chromatography;Surface plasmon resonance;Formulation;Comparability