A universal genome sequencing method for rotavirus A from human fecal samples which identifies segment reassortment and multi-genotype mixed infection

[摘要] BackgroundGenomic characterization of rotavirus (RoV) has not been adopted at large-scale due to the complexity of obtaining sequences for all 11 segments, particularly when feces are used as starting material.MethodsTo overcome these limitations, we developed a novel RoV capture and genome sequencing method combining commercial enzyme immunoassay plates and a set of routinely used reagents.ResultsOur approach had a 100% success rate, producing >90% genome coverage for diverse RoV present in fecal samples (Ct < 30).ConclusionsThis method provides a novel, reproducible and comparatively simple approach for genomic RoV characterization and could be scaled-up for use in global RoV surveillance systems.Trial registration (prospectively registered)Current Controlled Trials ISRCTN88101063. Date of registration: 14/06/2012

[发布日期] 2017-04-24

[关键词] Rotavirus A;Genomics;Phylogenetics;Genome sequencing;Reassortment;Co-infection;Antibody capture