HTQC: a fast quality control toolkit for Illumina sequencing data

[摘要] BackgroundIllumina sequencing platform is widely used in genome research. Sequence reads quality assessment and control are needed for downstream analysis. However, software that provides efficient quality assessment and versatile filtration methods is still lacking.ResultsWe have developed a toolkit named HTQC - abbreviation of High-Throughput Quality Control - for sequence reads quality control, which consists of six programs for reads quality assessment, reads filtration and generation of graphic reports.ConclusionsThe HTQC toolkit can generate reads quality assessment faster than existing tools, providing guidance for reads filtration utilities that allow users to choose different strategies to remove low quality reads.

[发布日期] 2013-01-31

[关键词] Work Thread;Sequencing Chip;Sequence Data Input;Quality Control Report;Quality Assessment Report