Lichenochemical Analysis and Cytotoxicity of Diploschistes ocellatus (Fr.) Norman

[摘要] Background and objectives: Diploschistes ocellatus (Fr.) Norman is a valuable lichen possessingvarious biological properties which has been traditionally used by indigenous people in southwest ofIran in the treatment of different disorders. The aim of the current study was to evaluate cytotoxicityof different fractions of D. ocellatus against breast cancer cell lines through MTT assay, as well aslichenochemical analysis of the most potent fraction. Also, ducking study was performed toinvestigate the isolated compounds-protein interactions. Methods: In this work, aqueous, acetone,chloroform, ethyl acetate, and methanol fractions of D. ocellatus were evaluated against three breastcancer cell lines (MCF-7, T-47D, and MDA-MB-231) via MTT assay. Furthermore, docking wasperformed using the routine method and default parameters of the AutoDock 4.2 software. Results:The acetone fraction depicted the most potent cytotoxicity and was candidate for lichenochemicalanalysis, leading to the isolation and identification of stictic acid and 2-(7'-hydroxy-3,5,6,8-tetramethyl-9-oxooxonan-2-yl) propanoic acid. Docking study of isolated compounds based on theinhibition of survivin, revealed desired interactions with that of amino acid residues. Conclusion:Based on the obtained results, D. ocellatus can be considered as a natural source of biologically activecompounds and complementary studies are in high demand.

[学科分类] 环境科学（综合）

[关键词] breast cancer;Diploschistes ocellatus;isolation;Lichens;Molecular docking simulation