Application of fluorescent potential-sensitive probes for the study of living blood and marrow cells

[摘要] Usage of membrane potential-sensitive probes is of great importance for assessing both the viability and functional integrity of the cells and their structural components (mitochondria, nuclei, cytoplasmic membranes, ion channels). Potential advantage of this approach includes studies of native viable cells in order to assess functional state of donor hematopoietic cells before transplantation as well as upon their storage and cultivation. These staining tools allow to assess the state of cellular bioenergetics, i.e., the balance between production and consumption of energy in living cells. The production of energy in mitochondrial structures ensures the cell viability, whereas its impairment leads to the development of different disorders and aging. The purpose of this literature review is to demonstrate the opportunities of using potential-sensitive probes to assess the viability of blood system cells and to study the state of their energy potential.

[学科分类] 肿瘤学

[关键词] Mitochondria;hematopoietic cells;membrane potential;electron transport;ion channels;energy status;cell viability;potential-sensitive probes.