Construction of transcription factor gene deletion library of Aspergillus luchuensis

[摘要] We selected 96 genes of Aspergillus luchuensis for the construction of a transcription factor gene deletion library. Of these, we successfully deleted 93 genes using Agrobacterium -mediated transformation (AMT) of A. luchuensis RIB 2604 ΔligD strains. We obtained only heterokaryonic strains after deletions of adaB , anBH1 , hacA , hapB , hsf1 , metR , and sonC gene, and additionally, could not obtain deletion strains for genes abaA and mcmA . The deletion strains will be available through our website (https://www.nrib.go.jp).

[学科分类] 微生物学和免疫学

[关键词] Aspergillus luchuensis;deletion library;transcription factor