Analysis of isolation of cerebral cortical neurons in rats by different methods

[摘要] The aim of this study was to find a way to efficiently separate neuronal cells from the cerebral cortex of adult rats, providing a reference method for rapid acquisition of neuronal cells from the adult rat brain. Fifteen SD rats were randomly divided into three groups, with five SD rats in each group. Then, neuron cells were isolated from the adult rat cerebral cortex by the grinding method, the trypsin method, and the collagenase II method, respectively. The expression of anti-NeuN in the neurons of each group was analyzed by flow cytometry. The acquisition rates and morphology of neurons of each group were observed by immunofluorescence staining. The grinding or collagenase II method is more suitable for rapid acquisition of neuronal cells from an adult rat’s cerebral cortex. The number of neuron cells obtained by the trypsin method were very few, so it is not convenient for later experiments.

[学科分类] 仪器

[关键词] Neurons;Grinding method;Collagenase II method;Trypsin method;Flow cytometry