Effect of peroxiredoxin 6 on total and progressive motility of human spermatozoa after cryopreservation

[摘要] Sperm cryopreservation is useful in assisted reproductive technology and male fertility preservation. However, freezing and thawing significantly reduces the total and progressive motility of human spermatozoa. In the present study, we explored the effects of peroxiredoxin 6 (PRDX6) on total and progressive motility of human spermatozoa after cryopreservation. Semen samples of 20 males with normal parameters were collected and frozen in media supplemented with different concentrations of PRDX6 (0 mM, 10 −5 mM, 10 −7 mM, and 10 −9 mM, respectively). Postthaw total and progressive motility of sperms were measured. The results showed that in comparison with 0 mM, the concentrations of 10 −5 mM, 10 −7 mM, and 10 −9 mM of PRDX6 all significantly improved total motility and progressive motility of sperms ( p < 0.05). The 10 −7 mM of PRDX6 showed the best performance. In conclusion, the supplementation of PRDX6 helps to maintain the total and progressive motility of human spermatozoa.

[学科分类] 仪器

[关键词] Peroxiredoxin 6;cryopreservation;sperm motility;progressive motility