Overloading of differentiated Caco-2 cells during lipid transcytosis induces glycosylation mistakes in the Golgi complex

[摘要] Overloading the intestine enterocytes with lipids induced alteration of the Golgi complex (GC; ) and could cause glycosylation errors. Here, using differentiated Caco-2 cells with the established 0[I] blood group phenotype (no expression of the blood antigens A and B [AgA, AgB] under normal conditions) as a model of human enterocytes we examined whether the overloading of these cells with lipids could cause errors in the Golgi-dependent glycosylation. We demonstrated that under these conditions, there were alterations of the GC and the appearance of lipid droplets in the cytoplasm. Rare cells produced AgA and AgB. This suggested that after overloading of enterocytes with lipids, AgA were mistakenly synthesized in individual enterocytes by the Golgi glycosyltransferases. These mistakes could explain why in the absence of AgA and AgB antibodies against them exist in the blood.

[学科分类] 仪器

[关键词] Golgi;Blood group antigens;Glycosylation errors;Enterocyte;Lipid transcytosis;Caco-2 cells