Isolation of Serratia fonticola Producing FONA, a Minor Extended-Spectrum β-Lactamase (ESBL), from Imported Chicken Meat in Japan

[摘要] Five novel strains of Serratia fonticola that produce FONA, a minor extended-spectrum beta-lactamase (ESBL), were isolated during routine surveillance of ESBL-producing Enterobacteriaceae in imported chicken meat in Japan in 2017 and 2018. These strains exhibited a clear ESBL phenotype in susceptibility tests carried out in the presence of clavulanic acid; however, all strains tested negative in a multiplex polymerase chain reaction assay used to detect TEM, SHV, and CTX-M β-lactamase genes. After identification of the bacterial species as S. fonticola , full length bla FONA genes were amplified and the DNA sequences were determined. The bla FONA genes from all 5 strains were different from those previously reported ( bla FONA-1 to bla FONA-6 ); they clustered close to one another but were distinct from previously reported bla FONA genes in a phylogenic analysis based on amino acid sequences.

[学科分类] 传染病学