Global metabolomic analysis of blood from mice infected with Brucella abortus

[摘要] To better understanding Brucella abortus infection, serum metabolites of B. abortus -infected and -uninfected mice were analyzed and twenty-one metabolites were tentatively identified at 3 and 14 days post-infection (d.p.i.). Level of most lysophosphatidylcholines (LPCs) was found to increase in infected mice at 3 d.p.i., while it was decreased at 14 d.p.i. as compared to uninfected mice. In contrast, acylcarnitines were initially reduced at 3 d.p.i then elevated after two-weeks of infection, while hydroxysanthine was increased at 14 d.p.i. in infected mice. Our findings suggest that the significant changes in LPCs and other identified metabolites may serve as potential biomarkers in acute phase of B. abortus infection.

[学科分类] 兽医学

[关键词] Brucella abortus infection;lysophosphatidylcholine;metabolomics profiling;serum;ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry