Synthesis of iron-oxide magnetic nanoparticles coated with dextran of varied molecular mass using a facile ball-milling method

[摘要] A simple ball-milling method was implemented to prepare dextran-coated superparamagnetic iron oxide nanoparticles (dextran-coated SPIONs, DSPIONs) by coating dextran (molecular mass, M dextran , 10, 40, 70 and 100 kDa) onto nanoparticles, which synthesised with co-precipitation methods. The size, saturation magnetisation and hydrodynamic diameters of the DSPIONs, composed of Fe 3 O 4 and γ-Fe 2 O 3 proved by X-ray diffraction and Raman spectra, were 11–14 nm, 55–65 emu g −1 and 200–300 nm using transmission electron microscope, superconducting-quantum-interference-device magnetometer and dynamic light scattering devices, respectively. The characteristic lines of surface coating were revealed in attenuated-total-reflection Fourier-transform infrared spectra. The saturation magnetisation and surface coating percentage (thermogravimetric analysis) of the ball-milled DSPIONs were independent on M dextran . The results were different from the statistical data from previous studies: DSPIONs using increasing M dextran were accompanied by decreasing saturation magnetisation and increasing surface coating in conventional co-precipitation protocols. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated the ball-milled DSPIONs exhibited low cytotoxicity in human hepatoma (SK-HEP1) cells, and the implementation of Prussian blue staining proved the DSPIONs were able to be uptaken by SK-HEP1. There was no obvious difference between the ball-milled DSPIONs with various M dextran . It demonstrated the ball milling process might be potential for SPION preparation in the biomedical field.

[学科分类] 计算机科学（综合）

[关键词] superparamagnetism;toxicology;nanoparticles;cellular biophysics;nanofabrication;magnetic particles;nanomedicine;nanomagnetics;cancer;biomedical materials;transmission electron microscopy;precipitation (physical chemistry);iron compounds;light scattering;Raman spectra;X-ray diffraction;Fourier transform infrared spectra;saturation magnetisation;thermal analysis;ball milling;nanocomposites;co-precipitated superparamagnetic iron oxide nanoparticles;biomedical applications;simple ball-milling method;dextran-coated superparamagnetic iron oxide nanoparticles;coating dextran;co-precipitation methods;saturation magnetisation;transmission electron microscopy;superconducting-quantum-interference-device magnetometer;dynamic light scattering devices;surface coating percentage;surface coating;conventional co-precipitation protocols;3-(4;5-dimethylthiazol-2-yl)-2;5-diphenyltetrazolium bromide assay;ball-milled DSPION;dextran-coated SPION;attenuated-total-reflection Fourier-transform infrared spectra;molecular mass;hydrodynamic diameters;thermogravimetric analysis;cytotoxicity;human hepatoma cells;Prussian blue staining;Fe2O3