Molecular cloning, characterization and expression analysis of BcHHP3 under abiotic stress in Pak - choi ( Brassica rapa ssp. Chinensis )

[摘要] In this study, BcHHP3 was isolated from Pak-choi; it has an open-reading frame (ORF) of 1044 base pairs, encoding 347 amino acids, a molecular weight of 39.35 kDa, isoelectric point (pI) of 9.08, an instability index of 48.35, and grand average of hydropathicity of 0.382. Multi-alignment and phylogenetic analysis showed that BcHHP3 bears a high similarity to AtHHP2. As predicted by SOMPA and SWISS-MODEL databases, the structure of the BcHHP3 protein is relatively stable and highly conservative. Real-time quantitative polymerase chain reaction (qRT-PCR) analysis showed that BcHHP3 was induced to co-express under cold and abscisic acid (ABA) stresses. The BcHHP3- GFP fusion protein was localized on the cell membrane and nuclear membrane. This work might be useful for future analysis of other HHP-like genes in Pak-choi.

[学科分类] 纳米科学和纳米技术

[关键词] Abiotic stress;expression analysis;Heptahelical protein 3 (HHP3);Pak-choi;subcellular localization