A styrene antioxidant NFA from riparian endophytic fungi enhances flooding tolerance in Arabidopsis

[摘要] Flooding is an important limiting factor for plant growth and development. Recent studies have shown that endophytic fungi symbiotic with extreme environmental plants can help hosts resisting various stresses. Previously, a styrene antioxidant NFA (Z-N-(4-hydroxystyryl) formamide) in riparian endophytic Aspergillus fumigatus was reported to effectively improve drought resistance in rice. To evaluate NFA’s ability to resist flooding of plants, we tested the antioxidant activity in yeasts, and investigated the flooding tolerance of Arabidopsis seedlings through fully submergence. The results showed that NFA could effectively alleviated oxidative damage by H2O2 in yeast and improved the flooding resistance of Arabidopsis. The tendency of oxidative parameters responded to flooding can be reversed by NFA, such as malondialdehyde, superoxide dismutase, ethanol dehydrogenase and NADPH oxidase. Expression analyses of NADPH oxidases and ROP protein families indicated that Atrboh D, Atrboh F and AtROP 11 may be involved in NFA-mediated flooding resistance.

[学科分类] 纳米科学和纳米技术

[关键词] Styrene antioxidant;NFA;endophytic fungi;flooding tolerance;Arabidopsis