Identification MdeSOS1 in Magnolia denudata and its function in response to salt stress

[摘要] In this study, annual Magnolia denudata seedlings were treated with a 200 mmol L−1 NaCl solution. Na+ content in its stems increased by more than about 24 times after 72 h and K+ content in leaves basically maintained in a steady state. MdeSOS1, isolated from M. denudata, comprised a 3453-bp ORF and included 12 transmembrane structures within its N terminal and a hydrophilic tail in its Cterminal. Its protein shared the identity of 68.3% and 62.7% at the peptide level with the homologue PeSOS1 and AtSOS1 respectively. The MdeSOS1 was significantly induced to upregulation in various tissues exposed to salt stress and improved the salt tolerance of Arabidopsis. Our results also revealed that the MdeSOS1-GFP fusion protein was located on the plasma membrane and MdeSOS1 encoded a salt-inducible plasma membrane Na+ /H+ antiporter, which provides a reference to improve the salt tolerance of Magnolia species by transgenic approaches.

[学科分类] 纳米科学和纳米技术

[关键词] Magnolia denudata;salt stress;SOS1;transgenic plants;Na+ /H+ antiporter