Use of infrared microspectroscopy to determine leaf biochemical composition of cassava in response to Bacillus subtilis CaSUT007

[摘要] The objective of this study was to investigate the growth stimulating properties of Bacillus subtilis CaSUT007 applied to cassava plants using fourier transform infrared (FTIR) microspectroscopy to monitor the production of cellular components involved in plant growth and development. Cassava stakes treated with CaSUT007 or sterile distilled water were germinated in soil. After incubation for 2 months, CaSUT007 treated plants had higher growth rate and greater biomass than the control. FTIR analysis revealed that the leaves of cassava plants treated with CaSUT007 display FTIR spectra changes in the epidermis and mesophyll tissue. These changes associated with proteins, lipids, and pectins, which are related to changes in plant cell growth and development. FTIR microspectroscopy can be used as a new tool to examine the biochemical changes within the plant tissue. This technique allows us to reveal structural chemical makeup and features of different tissue types.

[学科分类] 纳米科学和纳米技术

[关键词] Fourier transform infrared (FTIR);cassava;growth promotion;Bacillus subtilis;giochemical composition;hierarchical cluster analysis (HCA)