The impact of pRAP vectors on plant genetic transformation and pathogenesis studies including an analysis of BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1) -mediated resistance

[摘要] Crop improvement can be facilitated through efficient gene transfer, leading to pRAP plasmid development. Comparative hairy root transformation results from 24 previously published articles examining 29,756 roots show a 70% transformation efficiency. Average gene overexpression was 11.24-fold and −3.84-fold in RNAi roots. New studies show Glycine max BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1) overexpression leads to a 67% decrease in Heterodera glycines parasitism while BAK1-1 RNAi led to a 4.8-fold increase in parasitism. The results show pathogen associated molecular pattern triggered immunity (PTI) functions in the G. max-H. glycines pathosystem during defense. Consequently, the pRAP vectors have applicability for studying basic biology and defense in other agricultural plants including Manihot esculenta (cassava) , Zea mays (maize) , Oryza sativa (rice) , Triticum aestivum (wheat) , Sorghum bicolor (sorghum) , Brassica rapa (rape seed) , Solanum tuberosum (potato) , Solanum lycopersicum (tomato) , Elaes guineensis (oil palm) , Saccharum officinalis (sugarcane) and Beta vulgaris (sugar beet) since each have BAK1 homologs .

[学科分类] 纳米科学和纳米技术

[关键词] Plant;transformation;plasmid;pRAP15;pRAP17 overexpression;heterologous expression;ectopic expression;RNA interference (RNAi);crop;genetic;engineering;nematode;BRI1-ASSOCIATED RECEPTOR KINASE 1;BAK1