Screening of ethyl methane sulphonate mutagenized tef [ Eragrostis tef (Zucc.) Trotter] population identifies Al-tolerant lines

[摘要] About 15,000 M2 seeds of ethyl-methane-sulphonate (EMS)-mutagenized population were screened along with Al-tolerant and sensitive checks and the M0 variety. Strongly acidic soil with an external application of a toxic Al-solution and exposure to moisture stress was used to maximize selection pressure. Twenty-one M2 plants with root lengths of greater than the mean of the tolerant check were selected and planted for seed production. Candidate M3 plants were investigated for Altolerance and for morpho-agronomic traits under greenhouse and field conditions, respectively. Highly significant differences were observed for Al-tolerance between the candidate mutant lines and the M0 (P < .001), and between mutant lines and the sensitive check (P < .001). Similarly, significant differences were observed between the mutant lines for 16 of the 20 quantitative traits measured. This study is the first to report successful induction of enhanced Al-tolerance in tef by using EMS mutagenized population.

[学科分类] 纳米科学和纳米技术

[关键词] Aluminum;ethyl methane sulphonate (EMS);mutation breeding;tef