Cytosolic LOX overexpression in Arabidopsis enhances the attractiveness of parasitic wasps in response to herbivory and incidences of parasitism

[摘要] In response to herbivory by cabbage white butterfly ( Pieris rapae ) larvae, crucifer plants emit green leaf volatiles (GLVs) that attract Cotesia glomerata , a carnivorous natural enemy of the larvae. To artificially increase GLV production by crucifers, we created transgenic Arabidopsis that constitutively expressed cucumber cytosolic lipoxygenase ( CsLOX2 ). Transgenic Arabidopsis ( p35S::CsLOX2.6 and p35S::CsLOX2.14 ) infested with P. rapae larvae were more attractive to C. glomerata than wild type (wt) and produced more jasmonic acid. p35S::CsLOX2.14 had a higher incidence of parasitism of the larvae than did wt. Infested transgenic plants emitted more ( Z )-3-hexenyl acetate, ( E )-β-ocimene, ( Z )-β-ocimene, and DMNT than infested wt plants; ( Z )-3-hexenyl acetate is known to attract Cotesia wasps. Transforming cruciferous crops with cytosolic CsLOX2 could aid integrated pest management via tritrophic interactions.

[学科分类] 纳米科学和纳米技术

[关键词] Cotesia glomerata;green leaf volatiles (GLVs);herbivore-induced plant volatiles (HIPVs);lipoxygenase (LOX);Pieris rapae;transgenic plants