Molecular and biochemical markers of some Vicia faba L. genotypes in response to storage insect pests infestation

[摘要] Storage insect pests cause serious losses to all legume crops in both quality and quantity. This study was conducted to study some morphological characters and biochemical components in eight genotypes of faba bean to determine biochemical and molecular markers for resistance and susceptibility to infestation with stored insects. The results showed that chlorophyll content in leaves, phenol, tannin, peroxidase (POD), polyphenol oxidase (PPO), and protein content in leaves and seeds of faba bean plants either significantly or non significantly increased the effect of insect infestation in the resistance genotypes (L.551, L.512, L.153, NA112, and L.1) as compared with the susceptible genotypes (L.16 and T.W). Protein electrophoresis showed a wide variation between genotypes and determined some biochemical markers (sodium dodecyl sulfate poly acrylamide gel electrophoresis, SDS-PAGE). In addition, molecular genetic markers for stored insects’ resistance were obtained using inter-simple sequence repeat polymerase chain reaction (ISSR-PCR) analyses.

[学科分类] 纳米科学和纳米技术

[关键词] phenol;tannin;peroxidase;polyphenol oxidase;protein electrophoresis;ISSR-PCR